The participants in the double-blinded, crossover study were one golden retriever, five Labradors, and two English mastiffs. In some trials, each received a treat that included the three ingredients, as well as a placebo treat in others. In the in vitro investigation component, the researchers collected dog feces within 15 minutes of defecation, weighed it, and added the material to phosphate buffer. The resulting slurry was added to bottles with deformable and removable tops, and an agent was added to kill bacteria that would otherwise feast on the pungent contents. The researchers measured the pressure of the emitted gas and converted this into volume. A colorimetric assay determined the concentration of HS in parts-per-million (ppm).
But the dogs weren't finished after pooping. For five hours, for five consecutive days, each animal wore a jacket that held a perforated tube over the anus. The jacket also held a pump containing a ...
