What We Can Learn from the ELITE CONTROLLERS Infected nonprogressors are providing clues to the control, and potentially the eradication, of HIV By Gail Dutton FEATURE ARTICLES 25 Years with HIV ARTICLE EXTRAS Jeff Getty: Lessons in desperate measuresHow a risky experiment led to a new understanding of HIV HIV Shows ItselfA 1981 report in the MMWR marks the beginning The Impact of HIVIts progressions, 1981-2006 and beyond Why Monkeys Block HIVOld world monkeys don't

Jeff Getty in 1996 Jeff Getty: Lessons in desperate measures By Gail Dutton FEATURE ARTICLES The Elite Controllers of HIVGAIL DUTTON reports from San Francisco on how infected nonprogressors - also known as elite controllers - are providing clues to the control, and potentially the eradication, of HIV. 25 Years with HIV ARTICLE EXTRAS HIV Shows ItselfA 1981 report in the MMWR marks the beginning The Impact of HIVIts progressions, 1981-2006 and beyond Why Monkeys

Cloning DNA fragments using restriction enzymes is like flying from Seattle to New York via Phoenix.

Lab freezers are like mom's attic: cluttered with the property of people long gone.

Courtesy of UC Davis Medical Center  COMBI-KING: Kit Lam brings his expertise in combinatorial chemistry, an innovative technique for creating millions of new chemical compounds in just days, to the UC Davis Cancer Center. It's the new mix and match. In the computer industry, programmers refer to modularity--the ability to shuffle different sections of computer code to create new software. In the life sciences, the modules are peptides or molecular fragments that can be combined to yield

 TECHNICAL KNOCKOUT: A Cy3-labeled siRNA targeting B-actin was transfected into HeLa cells and protein expression was analyzed 96-hours later. Red, Cy3-labeled siRNA; Blue: DAPI-stained nuclei; Green, B-actin protein. (siRNA was prepared and labeled using Ambion's Silencer siRNA construction kit and labeling kit, respectively). RNA interference, or RNAi, is all the rage these days. According to the Web of Science database (ISI, Philadelphia), the number of articles on the topic jumped fr

Illustration: ©2002 Ned Shaw Editor's Note: With the gift-giving season upon us, The Scientist wanted to know what today's life scientists would be most grateful to receive this holiday season. We queried researchers throughout the world for ways to push life sciences to the next level, whatever that level might be. The final list has eight interrelated items. KNOWLEDGE INTEGRATION One of the most commonly expressed wishes was the desire for broader integration of knowledge among dis

Photo: Courtesy of Michelle Lizott Waniewski  DROP AND GIVE ME 20! Attendees at the 2002 NEB workshops labor through basic training. If you've ever been confused by the jargon of molecular biology, you are not alone. With biotechnology in the news day by day, it is easy to forget that for many of us--even for some readers of The Scientist--the tools, techniques, and concepts that undergird much of this research are foreign. To help keep up, some scientists are queuing up to learn the bas

Image: Courtesy of Rosetta Biosoftware  The Rosetta Resolver system's Image Viewer application showing an Affymetrix GeneChip probe array. There is a saying: "Be careful what you wish for, you just may get it." Biologists long pined for faster, more efficient ways to gather data; now they generate genomic information faster than they can assimilate it. The result: information overload. The solution: data mining. Though data mining is an ambiguous term, most definitions include the idea

Image: Courtesy of Invitrogen AIDING RESEARCH INSIDE AND OUT: Recombinases allow scientists to easily move DNA between vectors in vitro (as shown above, using Invitrogen's Gateway technology). In vivo, they enable researchers to knockout genes in specific tissues, and at specific developmental times, facilitating the study of otherwise "embryonic lethal" genes. Transgenic mice. Drought-tolerant canola. Medication-producing plants. And the still-unrealized potential of gene therapy. These