Researchers atBellBrookLabs have developed a high throughput screening (HTS) compatible biochemical assay to discover and advance small molecule modulators for the target cyclic GMP-AMP synthase (cGAS).TheTranscreenercGAMPcGASAssay directly detects cyclic GMP-AMP (cGAMP), the product of acGASreaction, allowing researchers to monitorcGASactivity with simple, mix-and-read fluorescent detection.
The presence of DNA in the cytosol of eukaryotic cells is an indicator of infection or cellular damage, and it elicits a strong innate immune response.cGAStriggers this response to cytoplasmic DNA by producing the dinucleotide second messengercGAMP, which activates the stimulator of interferon genes (STING) receptor leading to a type I interferon response. Recent studies suggest thecGAS-STING pathway is a promising area for therapeutics targeting autoimmune disease and cancer.
TheTranscreenercGAMPcGASAssayis a competitive immunoassay that uses a highly specific antibody forcGAMPand a fluorescent tracer.cGAMPdisplaces the tracer from the antibody resulting in a robust fluorescent polarization (FP) readout. Along with basiccGASresearch including enzymology and regulation, the new assay ...
