In the 22 December
Calling all binding sites
In the 22 December Science Ren et al. combine chromatin immunoprecipitation with DNA microarrays to identify all binding sites for two budding yeast transcription activators (Science 2000, 290:2306-2309). They start by breaking open cells, cross-linking bound protein to DNA, sonicating, and immunoprecipitating with an antibody against a particular transcription factor. The isolated DNA is amplified, and the abundance of the amplified fragments is compared with a whole genome amplification using

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