In total RNA sequencing experiments, many reads are not necessarily useful or desired for gene expression studies, including intronic reads from pre-mRNA or noncoding transcripts. Using target enrichment, researchers can hone in on important regions for gene expression and better detect low-abundance transcripts. Scientists recently tested an RNA exome panel that targets protein-coding isoforms for capture sequencing experiments. This targeted approach increased sequencing efficiency and identified structural variants, such as RNA fusions.
Download this poster from Twist Bioscience to learn how an RNA exome panel increases transcript signal with fewer reads, facilitating accurate gene expression profiling.