Discover How to Develop, Optimize, and Scale a Protocol for Generating Genetically Modified NK Cells
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Developing a Protocol for Generating Genetically Modified NK Cells

Natural killer (NK) cells target infected and oncogenic cells, but are difficult to work with in vitro. Discover novel approaches to producing genetically modified NK cells for cell therapy. 

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Natural killer (NK) cells rapidly identify and kill foreign, infected, and tumorigenic cells, making them promising candidates for cell therapy. However, compared to T cells, modifying primary NK cells is much more difficult. CRISPR can make the process smoother by making site-specific gene insertion more efficient. Indeed, scientists recently reported that electroporation with Cas9/ribonucleoprotein complexes followed by adeno-associated virus transduction resulted in efficient, stable, and functional editing of NK cells.

Download this application note from Thermo Fisher Scientific to discover a protocol for generating genetically modified NK cells, featuring upstream and downstream cell processing optimization and a combination of viral and nonviral payload delivery methods.

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