Pour off the aqueous phase when you use Phase Lock Get. Look Ma, No Pipette!
Pulling an aqueous phase off an organic phase is a truly hateful and hated task. Many an hour has been spent by many a researcher doing just that. With each extraction, a decision must be made-whether to go for purity, which often can only be achieved by leaving some aqueous behind, or for recovery by taking all you can, including some of the protein you're trying to get rid of. 5 Prime -> 3 Prime has come out with a product that will put all these worries behind you. The Phase Lock Gel (PLG) is a proprietary compound that creates a barrier between the aqueous and organic phases of an extraction, essentially trapping the organic phase below the PLG. You don't even need to use a pipette. The barrier is so durable that you can...

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