Don't Clone Those Genes!

If the expression products of a large number of different genes need to be rapidly analyzed, for instance during functional genomic research, consider the Transcriptionally Active PCR (TAP) Express™ Rapid Gene Expression kit from Gene Therapy Systems (GTS) Inc. of San Diego. TAP Express is the first commercially available system for the construction of transcriptionally active PCR fragments that can be expressed in mammalian cells. In this approach, researchers use nested PCR to generate t

Written byDeborah Fitzgerald
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"TAP should work with virtually any template DNA and is potentially useful for any researcher who is interested in quick and efficient transient protein expression in mammalian cells," comments Phil Felgner, chief scientific officer of GTS. Xiaowu Liang, TAP Express inventor and Molecular Biology department manager at GTS, adds that, "TAP Express is excellent for researchers who are interested in the expression screening of a gene. Better yet, researchers studying collections or families of genes can use the TAP Express kit to simultaneously produce proteins from a wide array of genes." One of TAP Express' advantages is that it is particularly amenable to high-throughput applications, because this methodology eliminates cloning steps on a large scale. Thus, TAP Express may prove to be especially useful for those in the functional genomics industry.

Scientists at GTS have demonstrated that protein expression from TAP fragments encoding any of a number of reporter genes ...

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