If the expression products of a large number of different genes need to be rapidly analyzed, for instance during functional genomic research, consider the Transcriptionally Active PCR (TAP) Express™ Rapid Gene Expression kit from Gene Therapy Systems (GTS) Inc. of San Diego. TAP Express is the first commercially available system for the construction of transcriptionally active PCR fragments that can be expressed in mammalian cells. In this approach, researchers use nested PCR to generate transcriptionally active fragments consisting of a proprietary promoter, the coding sequence of interest, and a viral terminator. TAP fragments, generated in just two PCR amplification steps, can then be directly transfected into cells or injected into animals. There is no need for cloning, transformations, or plasmid preps.
"TAP should work with virtually any template DNA and is potentially useful for any researcher who is interested in quick and efficient transient protein expression in mammalian cells," comments...
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