"Innovation is our mission," states Rockford, Ill.-based Pierce Chemical Company's Rick Chu. True to those words, Chu has applied Pierce's expertise in detergent chemistry to the development of a family of protein extraction reagents designed to rapidly and efficiently extract proteins from bacteria, yeast, and mammalian cell lines. Cell lysis, of course, is an important first step in recovering recombinant proteins or for providing samples for various assays. The new Pierce protein extraction reagents provide a gentle means of cell lysis that is not detrimental to the protein of interest.
The first-born member of this family, the B-PER Bacterial Protein Extraction Reagent, consists of a proprietary nonionic detergent in 20 mM Tris*HCl buffer (pH 7.5). In use, a 10- to 20-fold volume of B-PER™ Reagent is added to a bacterial pellet and shaken for 10 minutes. Soluble proteins are then recovered by a centrifugation step, and inclusion bodies are purified ...
