Expression Systems That Fly: Invitrogen's new Drosophila Expression System is fast, easy, and versatile

Functional expression of muscinaric receptors in Drosphila cells stably transfected with pMT/V5. Transient carbamylcholine induce Ca2+ uptake is shown by fluorescence of Fura-2 (Panel B), a Ca2+ sensitive dye.

Expressing proteins has never been easier than with Invitrogen's new Drosophila Expression System, DES^TM. DES takes you anywhere you want to go with protein expression and purification. With it you can make transient or stable transformants; by cotransfecting it with a hygromycin resistant vector, you can create transformants with hundreds of copies of your protein-bearing vector; it'll take your protein to the membrane, it'll secrete it, and it'll add a C-terminal tag to your protein. All these options are available along with the additional advantages of insect expression systems-room temperature, atmospheric growth, and growth in the absence of serum, further simplifying protein purification.

DES comes in four different forms. Two vectors contain the metallothionein promoter for inducible...