HeLa lysates were oxidized by the addition of H2O2 (hydrogen peroxide) for 0, 0.5 or 3.5 hours. Afterwards, cell lysates from the control or H2O2-treated samples were treated +/- dinitriphenylhydrazine, then separated by PAGE and transferred to a nitrocellulose membrane. Dinitrophenylated proteins were immunodetected using the anti-DNP antibody provided in the kit. The lanes contain: (1) dinitrophenylated molecular weight standards; (2) DNP-modified HeLa control cells; (3) DNP-modified, 0.5-hour H2O2-treated HeLa cells; (4) DNP- modified, 3.5 hour-H2O2-treated HeLa cells; (5) unmodified HeLa control cells; (6) unmodified HeLa, 0.5-hour H2O2-treatedHeLa cells. Reprinted with permission from Intergen.

Free radicals have been implicated in the oxidative modification of a wide variety of macromolecules, including nucleic acids, lipids, carbohydrates, and proteins. "During the last 5 to 6 years, increasing attention has been paid to the oxidation...

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