The Gibson Assembly® method has quickly become an invaluable tool for molecular cloning workflows due to its flexibility and efficiency. It allows for the cloning of multiple fragments directly into any vector without the use of restriction enzymes.
Gibson Assembly Primer-Bridge End Joining (PBnJTM) allows a single primer or primer pair with phosphorothloate-modified bases to bridge together the ends of DNA fragments.
Learn how to go from DNA fragments to complex plasmid constructs with this poster from SGI-DNA, a Synthetic Genomics Inc. Company.
