The Gibson Assembly® method has quickly become an invaluable tool for molecular cloning workflows due to its flexibility and efficiency. It allows for the cloning of multiple fragments directly into any vector without the use of restriction enzymes.
Gibson Assembly Primer-Bridge End Joining (PBnJTM) allows a single primer or primer pair with phosphorothloate-modified bases to bridge together the ends of DNA fragments.
Download this poster from The Scientist, sponsored by SGI-DNA, a Synthetic Genomics Inc. Company, to learn more about converting DNA fragments into complex plasmid constructs!
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