Give us the FACS

Thanks for your article on FACS.1 It was informative. How is the charge imparted to a specific droplet? By magic?Peter Calkins, PhDDepartment of Pathology pcalkins@bcm.tmc.eduResponse: The nozzle is actually charged, so as the droplet is formed a charge is applied to the droplet. Based on the sorting instructions, the droplet is charged either positively or negatively. The plates are always on during the sort.David MatsuyamaBecton Dickinson David_Matsuyama@bd.com

Written byPeter Calkins

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Thanks for your article on FACS.¹ It was informative. How is the charge imparted to a specific droplet? By magic?

Peter Calkins, PhD

Department of Pathology pcalkins@bcm.tmc.edu

Response: The nozzle is actually charged, so as the droplet is formed a charge is applied to the droplet. Based on the sorting instructions, the droplet is charged either positively or negatively. The plates are always on during the sort.

David Matsuyama

Becton Dickinson David_Matsuyama@bd.com

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