Infographic: Capturing a More Complete Picture of Expression

CITE-seq draws upon the unbiased nature of single-cell RNA sequencing to gather new insights about protein and gene expression within the same cell.

Written byDeanna MacNeil, PhD
| 1 min read

Register for free to listen to this article
Listen with Speechify
0:00
1:00
Share

Scientists mix single cells with ADTs and encapsulate them in droplets, along with microbeads. Cell lysis within the droplet releases mRNAs and antibody-derived tags (ADT) for microbead capture before PCR amplification, size separation, and single cell RNA sequencing (scRNA-seq).

Infographic showing how CITE-seq detects protein and gene expression in the same cell
© MESA SCHUMACHER

Read the full story.

Related Topics

Meet the Author

  • Deanna MacNeil, PhD headshot

    Deanna earned their PhD from McGill University in 2020, studying the cellular biology of aging and cancer. In addition to a passion for telomere research, Deanna has a multidisciplinary academic background in biochemistry and a professional background in medical writing, specializing in instructional design and gamification for scientific knowledge translation. They first joined The Scientist's Creative Services team part time as an intern and then full time as an assistant science editor. Deanna is currently an associate science editor, applying their science communication enthusiasm and SEO skillset across a range of written and multimedia pieces, including supervising content creation and editing of The Scientist's Brush Up Summaries.

    View Full Profile

Published In

June 2023 cover
Summer 2023

Divvying Up Duties

Bacteria cooperate to benefit the collective, but cheaters can rig the system

Share
You might also be interested in...
Loading Next Article...
You might also be interested in...
Loading Next Article...
Illustration of a developing fetus surrounded by a clear fluid with a subtle yellow tinge, representing amniotic fluid.
January 2026, Issue 1

What Is the Amniotic Fluid Composed of?

The liquid world of fetal development provides a rich source of nutrition and protection tailored to meet the needs of the growing fetus.

View this Issue
Skip the Wait for Protein Stability Data with Aunty

Skip the Wait for Protein Stability Data with Aunty

Unchained Labs
Graphic of three DNA helices in various colors

An Automated DNA-to-Data Framework for Production-Scale Sequencing

illumina
Exploring Cellular Organization with Spatial Proteomics

Exploring Cellular Organization with Spatial Proteomics

Abstract illustration of spheres with multiple layers, representing endoderm, ectoderm, and mesoderm derived organoids

Organoid Origins and How to Grow Them

Thermo Fisher Logo

Products

Brandtech Logo

BRANDTECH Scientific Introduces the Transferpette® pro Micropipette: A New Twist on Comfort and Control

Biotium Logo

Biotium Launches GlycoLiner™ Cell Surface Glycoprotein Labeling Kits for Rapid and Selective Cell Surface Imaging

Colorful abstract spiral dot pattern on a black background

Thermo Scientific X and S Series General Purpose Centrifuges

Thermo Fisher Logo
Abstract background with red and blue laser lights

VANTAstar Flexible microplate reader with simplified workflows

BMG LABTECH