Integrative genomics

In the Early Edition of the Proceedings of the National Academy of Sciences, Vamsi Mootha and colleagues describe an integrative genomics approach to identify a gene associated with the human cytochrome c oxidase (COX) deficiency, Leigh syndrome, French Canadian type (LSFC) (PNAS, DOI/10.1073/pnas.242716699, January 6, 2003).

LSFC is one of five autosomal recessive COX deficiencies; the other four are due to defects in genes encoding COX assembly factors. Mootha et al. combined experimental data from genomic, transcriptome and proteomic studies to identify the gene causing LSFC. They systematically analyzed potential genes in the LSFC candidate region that had been narrowed down to 2 megabase genomic region on chromosome 2p16-21. Neighborhood analysis of large-scale microarray data identified genes that are co-regulated with mitochondrial genes. This was combined with organelle-specific mass spectrometry proteomics data. Integrating these DNA, mRNA and protein-based data lead to the identification of a promising candidate gene LRPPRC (leucine-rich ...