Kinase Cartography

Courtesy of Zachary Knight  BEAUTIFUL CLEAVAGE: Trypsin cleaving a hypothetical protein into smaller fragments with C-terminal phosphorylated residues--a basis for phosphorylation mapping techniques. Proteins communicate with each other through posttranslational modifications, and locating modified sites is a key challenge in proteomics. Phosphorylation, the most common modification by far, is central to cell signaling, and knowledge of where and when proteins are phosphorylated could he

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Proteins communicate with each other through posttranslational modifications, and locating modified sites is a key challenge in proteomics. Phosphorylation, the most common modification by far, is central to cell signaling, and knowledge of where and when proteins are phosphorylated could help researchers decipher disease mechanisms, including those for cancer and diabetes.

Because protein modifications are not genetically encoded, it's difficult to track phosphorylation, says chemistry professor Kevan Shokat, University of California, San Francisco. A new technique that swaps phosphorylation sites for other more easily identifiable chemical species may enable fast and widespread identification, aiding researchers in decoding the posttranslational language of proteins. Proteomicists praise the elegance of the procedure, and the work earned first author Zachary Knight a grand prize in the 2002 Collegiate Inventors Competition.

A COURSE CHARTED The most commonly used method for mapping such sites is proteolytic digestion of the protein, followed by tandem mass spectrometry of ...

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