Scientists can use HTG's Multiplexed Molecular Profiling (MMP) technology to assess protein function and genetic polymorphisms, but the most popular application is measuring the RNA expression levels of a set of genes in multiple samples. Helen Brady, a scientist with San Diego-based Signal Pharmaceuticals, says her company has identified a number of interesting genes through array methods and is now using the HTG's ArrayPlate™ product "to look at the levels of a subset of genes under different conditions."
The technique works by adding linker oligonucleotides to a generic set of 16 "anchors" per well arrayed in a 4 x 4 grid. Each linker binds at one end to the anchor and at the other to a specific target molecule. Tissues or cells to be analyzed are grown, treated, and lysed in one microplate, to which nuclease protection reagents--synthetic sequences of DNA complementary to the RNA targets that protect them from ...
