User:
Pepper Schedin, University of Colorado Health Sciences Center, Denver
Project:
Relating life-cycle stages (such as pregnancy, lactation, and menopause) in the mammary gland stroma to tumor dormancy and progression
Problem:
Like Cukierman, Schedin looks at interactions of different ECMs with tumors. Schedin, though, extracts ECM from whole tissue; here, the resulting solution is too dilute to polymerize on its own - about 1/10 to 1/20th the concentration of Matrigel. Protocols to concentrate the ECM end up concentrating cellular contaminants along with it.
Solution:
Mixing the extracted ECM solutions 1:1 (volume to...
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