Molecular Biology

M.C. Schmidt, C.C. Kao, R. Pei, A.J. Berk, "Yeast TATA-box transcription factor gene," Proceedings of the National Academy of Sciences, 86, 7785-9, October 1989. Arnold Berk (University of California, Los Angeles): "TFIID binding to a TATA box initiates the ordered assembly of multiple transcription factors (TFs) required for initiation on eukaryotic promoters. However, TFIID resisted purification from higher organisms, preventing detailed studies of the process. We were interested in TFIID be

| 1 min read

Register for free to listen to this article
Listen with Speechify
0:00
1:00
Share

M.C. Schmidt, C.C. Kao, R. Pei, A.J. Berk, "Yeast TATA-box transcription factor gene," Proceedings of the National Academy of Sciences, 86, 7785-9, October 1989.

Arnold Berk (University of California, Los Angeles): "TFIID binding to a TATA box initiates the ordered assembly of multiple transcription factors (TFs) required for initiation on eukaryotic promoters. However, TFIID resisted purification from higher organisms, preventing detailed studies of the process. We were interested in TFIID because our work implied it was a target for adenovirus E1A transactivation. The breakthrough in this area came in 1988 with reports from Leonard Guarente and Phillip A. Sharp (Massachusetts Institute of Technology) (S. Buratowski, et al., Nature, 334:37-42, 1988), and Pierre Chambon (Institute of Biological Chemistry, Strasbourg, France) (B. Cavallini, et al., Nature, 334:77-80, 1988) that a protein from yeast could replace human TFIID for in vitro transcription with all other TFs and RNA polymerase from human cells. Based ...

Interested in reading more?

Become a Member of

The Scientist Logo
Receive full access to digital editions of The Scientist, as well as TS Digest, feature stories, more than 35 years of archives, and much more!
Already a member? Login Here
Illustration of a developing fetus surrounded by a clear fluid with a subtle yellow tinge, representing amniotic fluid.
January 2026, Issue 1

What Is the Amniotic Fluid Composed of?

The liquid world of fetal development provides a rich source of nutrition and protection tailored to meet the needs of the growing fetus.

View this Issue
Skip the Wait for Protein Stability Data with Aunty

Skip the Wait for Protein Stability Data with Aunty

Unchained Labs
Graphic of three DNA helices in various colors

An Automated DNA-to-Data Framework for Production-Scale Sequencing

illumina
Exploring Cellular Organization with Spatial Proteomics

Exploring Cellular Organization with Spatial Proteomics

Abstract illustration of spheres with multiple layers, representing endoderm, ectoderm, and mesoderm derived organoids

Organoid Origins and How to Grow Them

Thermo Fisher Logo

Products

Brandtech Logo

BRANDTECH Scientific Introduces the Transferpette® pro Micropipette: A New Twist on Comfort and Control

Biotium Logo

Biotium Launches GlycoLiner™ Cell Surface Glycoprotein Labeling Kits for Rapid and Selective Cell Surface Imaging

Colorful abstract spiral dot pattern on a black background

Thermo Scientific X and S Series General Purpose Centrifuges

Thermo Fisher Logo
Abstract background with red and blue laser lights

VANTAstar Flexible microplate reader with simplified workflows

BMG LABTECH