New drug hopes for RNAi?

Researchers linkurl:report;http://www.sciencemag.org/cgi/content/abstract/319/5863/627 that they have overcome one of the major roadblocks to using small interfering RNA (siRNA) therapeutically - they have developed a new method to linkurl:deliver siRNA;http://www.the-scientist.com/article/display/53307/ to silence genes in specific cells in vivo, according to this week's Science. "I'm really actually quite excited about the paper," said linkurl:John Rossi,;http://www.coh.org/Researchers/RossiJohn/RossiJohnResearch.htm who works on siRNA therapeutics and gene therapy at City of Hope in Duarte, California, and who was not involved in the research. "I really think this is a big breakthrough." In the same issue of the journal, two other groups of researchers linkurl:announce;http://www.sciencemag.org/cgi/content/abstract/319/5863/617 that they have linkurl:developed;http://www.sciencemag.org/cgi/content/abstract/319/5863/620 a technique using RNA interference to identify a new type of target for cancer therapeutics. Researchers have lauded the promise of siRNA as a tool for studying gene function as well as a potential therapeutic technique, but difficulties with delivering siRNA molecules into cells has dogged the field. linkurl:Motomu Shimaoka;http://www.cbrinstitute.org/labs/shimaoka/PI/PI.html and colleagues combined two approaches used in the past to create a nanoscale particle that can linkurl:target and transfect;http://www.the-scientist.com/article/display/53344/ leukocytes - a notoriously difficult cell type to transfect. The delivery vehicle consists of a liposome, or lipid vesicle, covered with two layers. A coating made of hyaluronan, common in the extracellular matrix of many tissues and often used as a biomaterial, stabilizes the molecule and maintains its tiny dimensions. A second layer uses antibodies to direct the molecule to its destination, where "the inside of the siRNA will release its payload quite fast," explained Daniel Peer, the study's lead author, based at Harvard. He noted that the technique was simple enough to be done in any lab. In the past, researchers have used liposomes for siRNA delivery, but the molecules tended to be large and toxic. Researchers have also used linkurl:antibodies to direct siRNAs;http://www.ncbi.nlm.nih.gov/pubmed/15908939?ordinalpos=11&itool=EntrezSystem2.PEntrez.Pubmed.Pubmed_ResultsPanel.Pubmed_RVDocSum to specific targets, but were only able to attach small numbers of siRNA to the antibody carriers. "The advance here is that you can package a greater amount of siRNA," said linkurl:Francis Szoka,;http://www.ucsf.edu/fszoka/ a drug delivery researcher at the University of California, San Francisco, who wrote the accompanying commentary to the study. What made that advance possible, said Rossi, was combining liposome and antibody approaches in a smart way. The researchers used their approach to knock down the gene for the protein linkurl:CyclinD1,;http://www.the-scientist.com/article/display/22358/ known for its role in cancer, and showed that blocking its function specifically in lymphocytes regulated cytokine levels and reduced inflammation in a mouse model of colitis. "The fact that this has potential in treating inflammatory diseases is really exciting," said Rossi. Shimaoka told The Scientist that his lab was looking into testing the technology in clinical trials "in the near future." But both Szoka and Rossi cautioned that linkurl:clinical applications;http://www.the-scientist.com/article/display/15190/ may be a ways off, because injecting antibodies creates the risk of harmful immune responses. In the other set of papers, linkurl:Steve Ellegde,;http://elledgelab.bwh.harvard.edu/ a Harvard geneticist, linkurl:Greg Hannon;http://www.cshl.edu/public/SCIENCE/hannon.html at Cold Spring Harbor, and colleagues used a linkurl:library of short hairpin RNA;http://www.the-scientist.com/article/display/52978/ (shRNA) to knock down the function of thousands of genes with known involvement in cell proliferation and survival. "Our philosophy," said Elledge, "is that [oncogenes] aren't the only linkurl:cancer targets;http://www.the-scientist.com/article/display/15213/ out there." Other genes which cancer cells need to survive might be involved in the many stresses a cancer cell undergoes, such as DNA damage, high reactive oxygen levels, aneuploidy, or the effects of chromosome deletions. "It was much easier to make a normal cell grow faster [than a cancer cell], so we're finding potential tumor suppressors in that category," said Elledge. The group also identified genes that play a larger role in cancer cells than normal cells, with distinct groups of genes providing a "lethality signature" for that type of cancer. "This is a pilot experiment - we only looked for a few thousand genes, but there's ten times more genes to explore," said Elledge, adding that the aim is to create a method that any lab can use. "Conceptually, we knew this kind of approach would work," said linkurl:Michael Green,;http://www.umassmed.edu/pgfe/faculty/green.cfm a cancer genetics researcher at the University of Massachusetts Medical School in Worcester, who was not involved in the research, but who uses the shRNA library developed by Elledge and Hannon in his own work. Such screens have been done on a smaller scale, he said, "but what they've done is put together an elegant set of technologies that allows this to be done on a genome-wide level and in many cell-types."

New drug hopes for RNAi?

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