New England Biolabs® Launches New NEBNext® Ultra™ II RNA Kits for Streamlined NGS Library Preparation with as Little as 5 ng of Total RNA

The kits are compatible with poly(A) mRNA isolation and ribosomal RNA (rRNA) depletion, and libraries can be constructed from a substantially broader input range (5 ng to 1 µg of total RNA) at high yields.

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New Ultra II technology addresses lower input amounts and challenging sample types for Illumina® next generation sequencing systems

IPSWICH, Mass., May 30, 2017 /PRNewswire/ -- New England Biolabs (NEB®) today announced the expansion of their NEBNext Ultra II NGS library preparation product portfolio to include kits for RNA. The strand-specific NEBNext Ultra II Directional RNA Library Prep Kit and the non-directional NEBNext Ultra II RNA Library Prep Kit are both designed for Illumina next generation sequencing systems, and substantially improve upon their predecessors. The kits are compatible with poly(A) mRNA isolation and ribosomal RNA (rRNA) depletion, and libraries can be constructed from a substantially broader input range (5 ng to 1 µg of total RNA) at high yields. Library complexity and transcript coverage are excellent even at low input amounts and with low quality samples such as formalin-fixed, paraffin-embedded (FFPE) RNA.

"As RNA-seq is adopted for a growing range of ...

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