New tool sheds light on cell imaging

In the same way it's hard to see the Milky Way in a major city, researchers sometimes struggle to see tagged structures because of natural background light emitted by cells. In this week's __Proceedings of the National Academy of Sciences__, however, researchers show that a new type of tag helps separate structures from the background. Gerard Marriott, from the University of Wisconsin-Madison, and colleagues found that using a blinking tag made it easier to spot cellular structures in live cel

Written byEdyta Zielinska
| 2 min read

Register for free to listen to this article
Listen with Speechify
0:00
2:00
Share
In the same way it's hard to see the Milky Way in a major city, researchers sometimes struggle to see tagged structures because of natural background light emitted by cells. In this week's __Proceedings of the National Academy of Sciences__, however, researchers show that a new type of tag helps separate structures from the background. Gerard Marriott, from the University of Wisconsin-Madison, and colleagues found that using a blinking tag made it easier to spot cellular structures in live cells. "Reading this paper is like, oh wow cool," said linkurl:Antoine Van Oijen,;http://www.the-scientist.com/article/display/54605/ a biophysicist at Harvard Medical School who was not involved in the research, "people who do live cell imaging, always have to deal with background." The living cell is a "dirty environment," explained Marriott -- more specifically, live cells give off their own fluorescence. Any tag used to mark a particular cellular structure has to be brighter than the background. This limitation makes it linkurl:difficult to detect;http://www.the-scientist.com/2008/7/1/65/1/ very small signals, like single molecules, or detect signals in cells with a large amount of natural fluorescence. The optical lock-in detection (OLID) method the authors describe uses a blinking signal to tag cellular structures, which makes it easier to spot the signal amidst the solid light source emitted by the background. "It's a little bit of a surprise that people haven't done this earlier," said Van Oijen; from the perspective of an electrical engineer, the technique is a bit obvious. "It's one of those things physicists have employed for years to detect a signal above the background." In these experiments, Marriott demonstrated how OLID staining of intact __Xenopus__ spinal cords and muscle in live zebrafish compared with conventional staining which was unable to pick up the fine detail of cellular structures. While most imaging labs have linkurl:the hardware;http://www.the-scientist.com/article/display/15428/ to perform these experiments, "the typical cell imaging labs won't be able to read this paper and do it tomorrow," said Van Oijen. While Marriott published the formula for converting the signal, no simple commercial software is available yet to pull the oscillating signal out of background automatically.
Interested in reading more?

Become a Member of

The Scientist Logo
Receive full access to more than 35 years of archives, as well as TS Digest, digital editions of The Scientist, feature stories, and much more!
Already a member? Login Here

Meet the Author

Share
Illustration of a developing fetus surrounded by a clear fluid with a subtle yellow tinge, representing amniotic fluid.
January 2026, Issue 1

What Is the Amniotic Fluid Composed of?

The liquid world of fetal development provides a rich source of nutrition and protection tailored to meet the needs of the growing fetus.

View this Issue
Skip the Wait for Protein Stability Data with Aunty

Skip the Wait for Protein Stability Data with Aunty

Unchained Labs
Graphic of three DNA helices in various colors

An Automated DNA-to-Data Framework for Production-Scale Sequencing

illumina
Exploring Cellular Organization with Spatial Proteomics

Exploring Cellular Organization with Spatial Proteomics

Abstract illustration of spheres with multiple layers, representing endoderm, ectoderm, and mesoderm derived organoids

Organoid Origins and How to Grow Them

Thermo Fisher Logo

Products

Brandtech Logo

BRANDTECH Scientific Introduces the Transferpette® pro Micropipette: A New Twist on Comfort and Control

Biotium Logo

Biotium Launches GlycoLiner™ Cell Surface Glycoprotein Labeling Kits for Rapid and Selective Cell Surface Imaging

Colorful abstract spiral dot pattern on a black background

Thermo Scientific X and S Series General Purpose Centrifuges

Thermo Fisher Logo
Abstract background with red and blue laser lights

VANTAstar Flexible microplate reader with simplified workflows

BMG LABTECH