Oxford Nanopore long-read sequencing helps researchers derive insights from difficult-to-read genomic sections and detect large structural variants. However, the technique uses high molecular weight (HMW) DNA, which is more fragile than DNA used for short-read sequencing and therefore requires sample and library prep optimization. Saliva, which contains primarily HMW DNA, may be a valuable source of DNA for Oxford Nanopore long-read sequencing.
Download this poster to see how rapid barcoding tagmentation-based (RBK) and ultra long-read (ULK) library prep methods are compatible with DNA from saliva collected in Oragene™ devices extracted with the prepIT™•L2P reagent, which enables the detection of large SVs, CNVs, and complex rearrangements with low infrastructure and high flexibility.
