The enzyme-linked immunosorbent assay (ELISA) is a plate-based technique that researchers use to quantify the levels of different molecules within a sample, including proteins, peptides, hormones, or antibodies. ELISAs rely on specific antibody-antigen interactions to capture target molecules, and as a result, this technique is versatile and highly selective. From clinical diagnostics to food testing, researchers customize ELISA protocols to the target molecule and sample type for optimal sensitivity, specificity, accuracy, and reproducibility.
Download this guide from Fortis Life Sciences to discover
- The basics of an ELISA protocol, including steps, formats, reagents, and data output
- How to prepare different types of samples for an ELISA
- The various options for troubleshooting and optimizing ELISA-based experiments