Tissue sample before (left) and after (right) treatmentFILLIPO PERBELLINIBy modifying CLARITY, an optical clearing technique first developed for brain tissue, to the heart, researchers at Imperial College London have created transparent cardiac tissue, according to a study published yesterday (July 12) in Scientific Reports.
CLARITY works by removing lipids from tissue, allowing light to penetrate. In recent years, researchers have applied this method various tissues, such as bone, and even to a whole mouse.
In the latest study, researchers combined CLARITY and DISCO (3-D imaging of solvent-cleared organs), another tissue-clearing technique, to create a new method, free-of-acrylamide SDS-based tissue clearing (FASTClear), to make both canine and human heart tissue transparent.
“Cardiac tissue is quite dense, using standard microscopy methods we are able to image the surface of the samples, about 20-30 microns from the surface,” coauthor Filippo Perbellin, a research associate at Imperial College London, says in a statement. “With more sophisticated methods we can achieve 50-80 microns depth but we were never able to image a whole myocardial ...
