Selected Genotyping Platforms | |||||
| System/Platform | Reaction Method | Pro | Con | Vendor | Relative Cost * |
| Luminex, Bio-Plex | ASPE (see figure), SBE, OLA | • Choice of chemistries for PCR • Multiplexing capability (up to 50-plex), especially for multiple variants from the same gene | • Optimization may be difficult • Requires dedicated instrument | Luminex, Bio-Rad | $$ |
| Molecular inversion probes & GeneChip | Probe hybridization | • Ideal for genome-wide studies • Can be customized for smaller projects • Can be outsourced | • Without outsourcing, requires costly capital investment | Affymetrix | $ |
| GoldenGate & BeadArray | ASPE & OLA | Illumina | $ | ||
| Infinium II & BeadArray | ASPE / SBE | Illumina | $ | ||
| TaqMan | FRET (see figure) | • Highly specific and sensitive • Simple closed-tube procedure | • Limited multiplexing • Relatively costly | Applied Biosystems | $$$ |
| High-resolution melting-curve analysis | Intercalating dye, hybridization probe (labeled or unlabeled) | • Rapid, inexpensive, suitable for multiplex... | |||
Interested in reading more?
Become a Member of
Receive full access to digital editions of The Scientist, as well as TS Digest, feature stories, more than 35 years of archives, and much more!
