Selection Perfection

The MACSelect Kk.II selects successfully transfected cells Low transfection efficiencies can obscure the results of even the most carefully planned experiment. Selection systems for enrichment of successfully transfected cells can help, but in the past they've relied on cotransfection of a selectable marker and your gene of interest (goi); this method only works when both plasmids enter the same cell. Miltenyi Biotec recently improved upon this by launching its MACSelect Kk.II Transfected Cell S

Written byAmy Francis

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The MACSelect K^k.II selects successfully transfected cells

Low transfection efficiencies can obscure the results of even the most carefully planned experiment. Selection systems for enrichment of successfully transfected cells can help, but in the past they've relied on cotransfection of a selectable marker and your gene of interest (goi); this method only works when both plasmids enter the same cell. Miltenyi Biotec recently improved upon this by launching its MACSelect K^k.II Transfected Cell Selection Kit.

Like other selection methods, the MACSelect Kk.II system first requires coexpression of your goi with a selectable cell surface marker. The selection marker is the mouse MHC class I H-2Kk protein, truncated to abrogate signal transduction. H-2Kk antibodies conjugated to a magnetic microbead bind to the surface marker, and H-2Kk expressing cells are retained in the magnetic field of a MACS column when placed in a MACS magnet. The major difference between the new MACSelect ...

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