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An image of neurons, astrocytes, and other glial cells acquired using fluorescence microscopy.
Troubleshooting Fluorescence Microscopy Experiments
Delve into the tactics used by scientists to overcome fluorescence microscopy’s greatest obstacles.
Troubleshooting Fluorescence Microscopy Experiments
Troubleshooting Fluorescence Microscopy Experiments

Delve into the tactics used by scientists to overcome fluorescence microscopy’s greatest obstacles.

Delve into the tactics used by scientists to overcome fluorescence microscopy’s greatest obstacles.

autofluorescence

Kaitlyn Sadtler standing in front of a laboratory bench
Spectral Flow Cytometry: Separating the Fluorophore Rainbow
Charlene Lancaster, PhD | Oct 24, 2023 | 3 min read
Spectral flow cytometry separates similarly emitting fluorophores and enabled Kaitlyn Sadtler to construct a 24-color rat panel for immunological analyses.
Lipofuscin autofluorescence was quenched using TrueBlack®, allowing effective visualization of glial cells (GFAP antibody stain, cyan) and cell nuclei (magenta) in human cerebral cortex cryosections.
Stepping Out of the Background
The Scientist Staff | Oct 2, 2023 | 1 min read
New innovations for reducing nonspecific fluorescence offer researchers clearer pictures and more multiplexing possibilities.
Lipofuscin autofluorescence was quenched using EverBrite TrueBlack® Hardset Mounting Medium®, allowing effective visualization of glial cells (GFAP antibody stain, cyan) and cell nuclei (magenta) in human cerebral cortex cryosections.
Stepping Out of the Background
The Scientist and Biotium | Jul 27, 2023 | 4 min read
New developments in non-specific fluorescence quenching offer researchers clearer pictures and more multiplexing possibilities.
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