What is Strip-EZTM? This new technology is incorporated into Ambion's StripAble¦ RNA Probe Synthesis Kits to optimize analysis of RNA and DNA populations using Northerns, Southerns, and related nucleic acid blots. Strip-EZ Technology (patent pending) employs modified nucleic acids that can be degraded by a reagent that does not affect standard RNA or DNA.
Standard RNA ProbeHigh sensitivity, Difficult to strip Strip-EZTM RNA ProbeHigh Sensitivity, Easy to strip controlStripped 2x with boiling SDScontrolStripped 1x with Strip-EZ Probe Degradation Buffer Comparison of RNA probes and Ambion Strip-EZTM probes: Four identical Northern blots were generated using a dilution series (3, 1, 0.3, and 0.1 micrograms) of mouse lung RNA. The blots were paired and each pair was probed with 5 x 106 cpm of RNA or Strip-EZ RNA probes specific to the mouse ß-actin mRNA using Ambion's NorthernMaxTM Kit. (RNA and Strip-EZ RNA probes were hybridized at 65ºC.) One of the blots ...
