Watch Your Transfections ... Literally

There is always room for improvement, even for products that were good from the start. Take cationic liposome-based reagents, for example. Researchers have widely used such compounds to efficiently introduce nucleic acids into cells for over a decade. Qbiogene of Carlsbad, Calif., recently improved on this technology with the introduction of in vivo and in vitro FluoroFectin™ reagents, developed in the laboratory of Yechezkel Barenholz of the Hebrew University in Jerusalem. FluoroFectin

Written byAileen Constans

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There is always room for improvement, even for products that were good from the start. Take cationic liposome-based reagents, for example. Researchers have widely used such compounds to efficiently introduce nucleic acids into cells for over a decade. Qbiogene of Carlsbad, Calif., recently improved on this technology with the introduction of in vivo and in vitro FluoroFectin™ reagents, developed in the laboratory of Yechezkel Barenholz of the Hebrew University in Jerusalem.

FluoroFectin reagents are fluorescently tagged, cationic liposome-based reagents that, unlike conventional transfection reagents, allow visualization of transfection events. They are available in a variety of formulations: dioleoyltrimethylammonium propane (DOTAP); DOTAP/dioleoylphosphatidylethanolamine (DOPE), for transfection of insect cells; and DOTAP/Cholesterol, for enhanced transfection efficiency. A specialized version of DOTAP/Cholesterol is available for in vivo research. FluoroFectin reagents offer an alternative to reporter gene assays and can be used in high-throughput fluorescence-activated cell-sorting (FACS) analysis. According to product manager Jane MacDougall, the ...

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