User: John Yates III, professor of chemical physiology, The Scripps Research Institute, La Jolla, Calif. Project: Identifying insulin-signaling targets in Caenorhabditis elegans Related Articles Peak Addition Tips for Quantifying Mass Spec Easy numbers Multiplex counts Label-free Metabolic power Problem: Yates needed a rapid and accurate way to measure the change in abundance of three specific targets over time and growth temper

User: Anthony Whetton, professor of cancer cell biology, University of Manchester, UK Project: Measuring proteomic changes induced by a panel of oncogenic tyrosine kinases in cultured cells Related Articles Peak Addition Tips for Quantifying Mass Spec Easy numbers Clean targeting Label-free Metabolic power Problem: Quantifying samples serially takes a long time and adds variability to the data, so Whetton wanted to measure

User: Ruedi Aebersold, professor for molecular systems biology, Swiss Federal Institute of Technology, Zurich Project: Breast cancer biomarker discovery in human serum Related Articles Peak Addition Tips for Quantifying Mass Spec Easy numbers Clean targeting Multiplex counts Metabolic power Problem: Aebersold needed a technique that could look for differentially expressed proteins in hundreds of clinical samples.

User: Matthias Mann, professor, Department of Proteomics and Signal Transduction, Max-Planck Institute for Biochemistry, Martinsried, Germany Project:Measuring proteome changes in cultured Drosophila cells from RNAi-induced knockdown of specific gene products Related Articles Peak Addition Easy numbers Clean targeting Multiplex counts Label-free Problem: Mann wanted to cut down on side reactions that can plague chemical and

Use spike-in controls for absolute quantitation. For most biological applications, relative quantitation should suffice. Sometimes, though, absolute numbers matter. The solution: spike-in controls. Add a known quantity of an isotopically labeled form of the desired peptide (or close surrogate), and use its abundance to correlate peak height with molecular abundance. This peptide will behave identically with the peptides in your samples during sample

Turning Tobacco into Therapies By Jeffrey M. Perkel RELATED ARTICLES Innovative Technology Daniel Skovronsky: Scientist and leader Biofuel: The Potential Magic Bullet Britton Chance: Still searching for answers Art Caplan: Penn's renowned ethicist Technology Roundup The Delaware Technology Park in Newark just may be the site of the next revolution. There, in a two-story building aptly named "9 Innovation Way," the Fraunhofer USA Center for Mo

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