In the 3 May Nature, Fred Gage and colleagues of the Salk Institute in La Jolla, California report the successful isolation and propagation of neural progenitor tissue from post-mortem brain tissue (Nature 2001, 411:42-43).Two hours post mortem brain tissue was removed from an 11-week-old neonate and a 27-year-old adult. The samples were placed in cold antibiotic-containing Hank's buffered salt solution and processed for cell culture 3 hours later. Representative sections of the hippocampus, ven
In the 3 May Nature Oliver Guillin and colleagues report that the expression of the dopamine D3 receptor is regulated by the brain-derived neurotrophic factor (BDNF), a protein once thought to be needed simply for the proliferation and survival of neurons (Nature 2001, 411:86-89).D3 receptors are expressed mainly in an area of the brain known as the shell of the nucleus accumbens. Neurons from the ventral tegmental area (VTA), which produce both dopamine and BDNF, connect to neurons of the nucle
A mouse strain has been generated in which K-ras alleles are activated at random, mimicking the sporadic occurrence of K-ras mutations in human cancers.
Three independent studies quash the theory that experimental polio vaccines administered in Central Africa in the 1950s were the origin of the AIDS pandemic.
Discover how single-molecule Fiber-seq is revealing unexpected mechanisms of transcription factor regulation, chromatin accessibility, and essential gene activation at CpG-island promoters.
Explore how emerging biomarker research and ultrasensitive multiplex proteomics are revealing molecular signatures associated with neurodegeneration, inflammation, and disease progression in amyotrophic lateral sclerosis (ALS).
Explore how in vivo CAR-neutrophil engineering and advanced light-sheet imaging are opening new avenues for studying and targeting glioblastoma and other solid tumors.
Explore practical strategies for designing, optimizing, and validating qPCR assays to improve specificity, reproducibility, and confidence across routine and high-throughput gene expression studies.
Automation can help to increase efficiency and reproducibility, but often comes at a significant cost. The ASSIST PLUS pipetting robot streamlines workflows at an affordable price, offering accessible automation with minimal programming requirements.
Corning® Cell Strainers are sterile, easy-to-use devices for rapidly isolating primary cells to consistently obtain a uniform single-cell suspension from tissues. Applications include stem cells and primary cells.
Real time imaging shouldn't outlast your cell surface stain. CellBrite® Steady: cell surface staining kits with fluorescent membrane dye plus enhancer for imaging live cell surface for several hours to days.
