Microarray analysis, which allows scientists to analyze expression patterns of thousands of genes simultaneously, is the tool of the new millennium; no laboratory interested in genes and gene function can afford to ignore this technique. The technology, however, can be expensive and difficult to master, thus leaving some laboratories wary of investing in it. Now Amersham Pharmacia Biotech of Piscataway, N.J., offers the CyScribe™ First Strand cDNA Labeling Kit, designed to "enable even first time microarray experiments to be successful," says Jim Grobholz, North American regional product manager for the CyScribe kit.
Microarray slide hybridized with Cy3 and Cy5-labeled probes Microarray analysis uses fluorescently labeled cDNA probes prepared from cellular RNA. When comparing RNA from two different samples, such as control and diseased tissue, two different fluorescent labels are utilized. Researchers must feel confident that changes in fluorescence actually represent alterations in gene expression and not an artificial enhancement of ...
