 Process of proteins flowing through channels of Continuous Bed. |
"The faster you can load the column without losing any resolution, the better off you're going to be. In all I've done, [sample loading] has always been the limiting step," says Anthony David Couvillon, lab manager in the division of signal transduction at Beth Israel Deaconess Medical Center in Boston, Massachusetts. Loading a column often takes longer than the actual run.
With that in mind, Bio-Rad has introduced its new UNO ion exchange columns for biomolecule purification. They're packed with a novel "Continuous Bed" matrix that promises greatly increased flow rates-and that makes sample loading a lot less painful says Couvillon. He started loading the column at his typical flow rate of 1/2 ml per minute, slowly raised ...
