At the annual meeting of the American Society for Cell Biology (ASCB) in Philadelphia, PA, USA, from December 6 to 10, 2014, Leica Microsystems offers visitors a preview of its latest development: a light sheet module as an optional add-on to the Leica TCS SP8 confocal microscope for the observation of developing organisms in real time and 3D. The synergy of light sheet and confocal microscopy opens novel fields of application.

Light sheet microscopy is a gentle method of imaging light-sensitive samples or fast biological processes in whole organisms, because the specimen is illuminated in a single plane, which reduces phototoxic effects and protects the sample. By moving the sample along this plane, imaging of three-dimensional structures is possible. A high-speed camera allows the imaging of fast cellular processes. Light sheet microscopy is ideal for applications in developmental biology.

Light sheet microscopy usually requires a dedicated optical setup on a...

The light sheet module is fully integrated into the Leica TCS SP8 modular imaging platform and expands its imaging options. Every inverted Leica TCS SP8 can be upgraded to a light sheet microscope. The system can be tailored to the users’ specific needs and additional functionality such as STED super-resolution, multiphoton microscopy, advanced lasers or quantitative imaging can be added whenever needed.

More confocal innovations by Leica Microsystems will be presented at the ASCB meeting in Philadelphia, booth #801: Visitors can also see a pulsed STED laser at 775 nm, which achieves sub 30 nm resolution, as well as the universal hybrid detector for quantitative FCS, FLIM, FLCS, and super-sensitive imaging.

Leica Microsystems is a world leader in microscopes and scientific instruments. Founded as a family business in the nineteenth century, the company’s history was marked by unparalleled innovation on its way to becoming a global enterprise.
Its historically close cooperation with the scientific community is the key to Leica Microsystems’ tradition of innovation, which draws on users’ ideas and creates solutions tailored to their requirements. At the global level, Leica Microsystems is organized in three divisions, all of which are among the leaders in their respective fields: the Life Science Division, Industry Division and Medical Division.
The company is represented in over 100 countries with 6 manufacturing facilities in 5 countries, sales and service organizations in 20 countries, and an international network of dealers. The company is headquartered in Wetzlar, Germany.

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