GeniePrepTM Kit Ambion’s GeniePrep Kit demonstrates versatility in purifying DNA away from different buffers and contaminants including proteins, carbohydrates, RNA, oligonucleotides and DNA linkers. This one kit is designed to isolate linear and circular double-stranded DNA from a variety of sources: from E. coli, from a variety of enzyme reactions, such as restriction digestions and PCR, and DNA fragments can be isolated directly from low-melt agarose. DNA purified by GeniePrep is suitable for restriction enzyme digestion, DNA sequencing, PCR, in vitro transcription, ligation, and transformation. GeniePrep can also be used to remove unincorporated, free radioactive and nonisotopically labeled nucleotides and primers away from DNA in random-primed labeling reactions. Depending on the application, the GeniePrep procedure takes from 10 to 30 minutes.

The GeniePrep Kit utilizes a glass fiber pad premounted in a spin column that preferentially binds DNA. Each of the applications follows a simple, general protocol. The...

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