New England Biolabs (NEB®) today announced the release of the NEBNext® Single Cell/Low Input RNA Library Prep Kit for Illumina®. This new kit incorporates an optimized template switching protocol— as well as NEB's Ultra™ II FS enzymatic DNA fragmentation technology— to produce full-length transcript sequence-ready libraries from single cells and ultra-low input RNA.
"Studies based on population analyses using thousands of cells disguise the potentially significant biological variations among sub-populations of cells and in individual cells, and the benefits of sequencing the transcriptomes of these smaller samples is clear," said Fiona Stewart, NEBNext Portfolio Manager at NEB. "However, there is a need for more sensitive, reliable and affordable products for library preparation for single cell and ultra-low input RNA samples, in order to increase the breadth of transcripts detected, and the reliability of that transcript detection, as well as enabling distinction between sample variation and experimental variation."
"To address this ...
