New Pluripotent Stem Cell Media Eliminates Need for Daily Feeding

Media allows two full-day breaks without changes to set up or protocol.

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A new pluripotent stem cell (PSC) medium is designed to offer researchers unprecedented schedule flexibility, allowing two-day breaks from culture feeding without having to make changes to current workflows or compromise culture quality. Researchers can save time and reduce sample handling, thereby decreasing the risk of contamination.

The Gibco Essential 8 Flex Medium, from Thermo Fisher Scientific, will debut at the International Society for Stem Cell Research (ISSCR) 2015 Annual Meeting, June 24-27, 2015 at the Stockholmsmässan Exhibition & Convention Center, Stockholm, Sweden Booth B06:32 and B06:33.

Culturing PSCs is a technical and time-consuming process. Many stem cell media components, such as fibroblast growth factor 2 (FGF2), lose activity at physiologically relevant temperatures and, therefore, stem cell cultures must be fed daily to preserve pluripotency and tri-lineage differentiation potential. To achieve this, scientists typically must work through the weekend or delegate feeding steps to other scientists, which can introduce unnecessary ...

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