AMSBIO has introduced two new products to assist the increasing number of scientists adopting organoid culture as a physiologically relevant model for organ development.
These new tools build upon the widespread use of AMSBIO’s BME2 ‘organoid matrix’ by leading research groups worldwide. BME 2 is the extracellular matrix of choice for growing and supporting organoid cultures that are increasingly being adopted in drug screening applications. A new R-Spondin1 cell line has been developed that provides a cost-effective solution by which labs can obtain a long-term supply of R-Spondin1 (RSPO1), which is a critical ingredient used in the maintenance and proliferation of mouse and human organoid progenitor stem cells. R-Spondins positively regulate Wnt/beta-catenin signaling, most probably by acting as a ligand for LGR4-6 receptors and an inhibitor for ZNRF3. Our 293T cell line is stably transfected to express murine RSPO1 with an N-terminal HA epitope tag and fused to a C-terminal ...
