Next-Generation Sequencing Oncomine Focus Assay for Oncology Research

Targeted, multi-biomarker assay enables simultaneous detection of single nucleotide variants, indels, copy number variants and gene fusions across 52 genes relevant to solid tumors


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Translational and clinical research laboratories now have access to a multi-biomarker, next-generation sequencing (NGS) panel that includes 52 solid tumor genes associated with current oncology drugs and published evidence. The Oncomine Focus Assay by Thermo Fisher Scientific (NYSE: TMO) is being featured at the American Association for Cancer Research conference taking place April 18-22 at the Pennsylvania Convention Center in Philadelphia.

The Oncomine Focus Assay allows concurrent analysis of DNA and RNA, enabling sequencing of 35 hotspot genes, 19 genes associated with copy number gain and 23 fusion genes, all in a single workflow using the Ion PGM System. The assay also leverages Ion AmpliSeq technology’s low DNA and RNA sample input requirements from FFPE tissue (10ng extracted nucleic acid per reaction for a total of 20ng per sample) to enable accurate and reliable sequence analysis across a large range of tumor sample types, including small biopsies and fine needle ...

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