One to Grow On

Dishes, Flasks and Roller Bottles. Plates Sarstedt's tissue culture dishes A cell is considered cultured once it can adhere to a substrate or remain in suspension and proliferate. A primary culture is derived either from an outgrowth of migrating cells from a tissue fragment or from mechanically or enzymatically dissociated tissue. Monolayer cells capable of proliferation will grow to confluence, and cells that are contact inhibited will stop growing at a certain cell density. Transformed cells

Written byMichelle Vettese-dadey

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Dishes, Flasks and Roller Bottles.
Plates

Sarstedt's tissue culture dishes

A cell is considered cultured once it can adhere to a substrate or remain in suspension and proliferate. A primary culture is derived either from an outgrowth of migrating cells from a tissue fragment or from mechanically or enzymatically dissociated tissue. Monolayer cells capable of proliferation will grow to confluence, and cells that are contact inhibited will stop growing at a certain cell density. Transformed cells often become anchorage independent and can grow in suspension.¹

From the earliest attempts to culture cells, glass was selected as a growth surface because of its optical properties and because it seemed to carry the correct charge for cell attachment and growth. However, glass vessels required base and acid washes to remove protein deposits and to restore the hydrophilic properties of the surface. Additionally, tissue culture-grade glassware required a lengthy washing regimen, autoclaving, and ...

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