Six Degrees of Separation

HPLC Products Characteristics of commonly used columns for biomolecules Rheodyne's automated HPLC column selector Simplification might be the single common goal of most scientific disciplines. Whether the entity of interest is an equation, a reaction, or an organism, the details need to be defined if the complexity of the whole picture is to be understood. In the research laboratory, many techniques exist for separating complex biological mixtures to attain the simple facts. Some of these meth

Written byDebra Swanson

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HPLC Products

Characteristics of commonly used columns for biomolecules

Rheodyne's automated HPLC column selector

Simplification might be the single common goal of most scientific disciplines. Whether the entity of interest is an equation, a reaction, or an organism, the details need to be defined if the complexity of the whole picture is to be understood. In the research laboratory, many techniques exist for separating complex biological mixtures to attain the simple facts. Some of these methods include extraction, electrophoresis, filtration, and chromatography.

Chromatography has been a trusted friend to researchers for many years. The term "chromatography" originated with the method first described in 1906 by Mikhail Tswett, a Russian botanist/physical chemist, who separated colored chloroplast pigments in a calcium carbonate tube.1 Following a 25-year period of relative latency, the methodology achieved wide acceptance in the 1930s when R. Kuhn, E. Lederer, and A. Winterstein resolved xanthophyll pigments into their isomeric ...

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