CLONTECH's SMART RACE cDNA amplification process diagram On your mark, get set, go! And the RACE is on to obtain that potentially elusive full-length cDNA for characterizing the best gene ever. In the molecular biology arena, RACE refers to rapid amplification of cDNA ends, a PCR-based cloning strategy used to obtain clones representing transcripts of the int-2 gene, expressed at low abundance in the early mouse embryo.1 Traditional methods of cDNA production, such as constructing and screenin
 CLONTECH's SMART RACE cDNA amplification process diagram |
Early RACE protocols did minimize the time expenditure problem by eliminating steps, but other shortcomings remained: high background noise due to the generation of nonspecific PCR products, which often required a second round of PCR with nested primers to achieve greater specificity; ...
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