Tailored Arrays

DNA array-based technologies are widely used in biological research, most commonly to measure differential gene expression--that is, comparing the relative levels of RNA transcripts in different cell or tissue samples.1,2 In a common scenario, researchers label cDNA targets prepared from control and experimental samples with two different fluorescent dyes. They then hybridize these two samples simultaneously to a single microarray bearing tens of thousands of oligonucleotide probes. Finally, the

| 10 min read

Register for free to listen to this article
Listen with Speechify
0:00
10:00
Share

Investigators can either generate their own arrays in-house if they have the requisite instrumentation, or alternatively, custom-order them from companies that provide this service. However, growing numbers of "stock," premade arrays are becoming commercially available. Owing to their prefabricated nature, these products offer less flexibility in experimental design. However, purchasing arrays "off the shelf" may prove to be an economical and timesaving option for a variety of applications.

Premade arrays vary in the relative number and nature of the gene probes, spot size, type of support used, and the method used to make the array. There is great diversity in the numbers of genes evaluated; premade arrays can contain from tens to thousands of probes. "Microarrays" contain spots of relatively small diameter, and utilize supports that hinder spot spreading (e.g., glass or plastic). These types of supports allow one microarray to be simultaneously hybridized with different fluorescently labeled samples, although ...

Interested in reading more?

Become a Member of

The Scientist Logo
Receive full access to digital editions of The Scientist, as well as TS Digest, feature stories, more than 35 years of archives, and much more!
Already a member? Login Here

Meet the Author

  • Deborah Fitzgerald

    This person does not yet have a bio.

Published In

Share
Image of a woman in a microbiology lab whose hair is caught on fire from a Bunsen burner.
April 1, 2025, Issue 1

Bunsen Burners and Bad Hair Days

Lab safety rules dictate that one must tie back long hair. Rosemarie Hansen learned the hard way when an open flame turned her locks into a lesson.

View this Issue
Faster Fluid Measurements for Formulation Development

Meet Honeybun and Breeze Through Viscometry in Formulation Development

Unchained Labs
Conceptual image of biochemical laboratory sample preparation showing glassware and chemical formulas in the foreground and a scientist holding a pipette in the background.

Taking the Guesswork Out of Quality Control Standards

sartorius logo
An illustration of PFAS bubbles in front of a blue sky with clouds.

PFAS: The Forever Chemicals

sartorius logo
Unlocking the Unattainable in Gene Construction

Unlocking the Unattainable in Gene Construction

dna-script-primarylogo-digital

Products

Metrion Biosciences Logo

Metrion Biosciences launches NaV1.9 high-throughput screening assay to strengthen screening portfolio and advance research on new medicines for pain

Biotium Logo

Biotium Unveils New Assay Kit with Exceptional RNase Detection Sensitivity

Atelerix

Atelerix signs exclusive agreement with MineBio to establish distribution channel for non-cryogenic cell preservation solutions in China

Green Cooling

Thermo Scientific™ Centrifuges with GreenCool Technology

Thermo Fisher Logo