The Mutation Sensation: Cleavase Fragment Length Polymorphism from Third Wave Technologies

p53 GENE MUTTION: Exons 5 and 6 of the p53 gene of five individuals were amplified and examined by Cleavase® Fragment Length Polymorphism (CFLP®) Analysis. The pattern marked "WT" is from an individual known to have a normal p53 gene.

For those of you reluctant to put a down payment on an automated sequencing apparatus, pay heed to an alternative that may fit your applications. Third Wave Technologies (Madison, Wis.) recently introduced a procedure for identifying generic sequences and mutations. Dubbed Cleavase Fragment Length Polymorphism (CFLP"), the method employs the proprietary enzyme Clevase I to cleave DNA into a series of fragments which appear as bands on sequencing gels. Utilising this method, double-stranded DNA obtained via PCR is denatured and then cooled in low-salt buffer. Under these conditions the denatured DNA strands form hairpin-like structures. Cleavase I cuts at the 5' region of the hairpin secondary structure...