Now that we have the whole human genome sequence, the challenge remains to identify all the genes and transcripts hidden within it. In the October 9
The approach being used exploits open reading frame expressed sequence tags ORESTES. This differs from the conventional expressed sequence tag (EST) strategy in that it provides sequence information along the whole length of each transcript, rather than just the ends. The method involves low-stringency PCR to produce cDNA libraries, samples of which are then sequenced.
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