It has been thought that genes involved in thiamine (vitamin B1) biosynthesis might be controlled by a thiamine pyrophosphate-dependent sensor/regulatory protein, but no such protein has been shown to exist. In October 17
Winkler et al used β-galactosidase translational fusion constructs from which they generated DNA templates and then RNA fragments. The resulting RNAs were analysed using a structure probing process to reveal whether the RNAs undergo structure modulation upon binding of ligands.
Using this system Winkler et al. observed that the mRNAs encoding enzymes involved in thiamine biosynthesis in