Annotation by SAGE

Most of us are acutely aware of the limitations of current in silico methods for predicting genes in the human genome. In the May issue of Nature Biotechnology, Saurabh Saha and colleagues at the Johns Hopkins Medical Institutions describe an experimental approach for gene discovery and genome annotation (Nature Biotechnol 2002, 19:508-512).

The method is an adaptation of the SAGE (serial analysis of gene expression) technology developed in the Vogelstein/Kinzler lab at Johns Hopkins. Compared to SAGE, the new 'LongSAGE' method uses a different type IIS restriction endonuclease (called MmeI) to create longer 21 base-pair 'tags', and the longer tag length should allow unique assignment to genomic loci.

Saha et al. analyzed 28,000 transcript tags expressed by a colon cancer cell line and found that the majority could be uniquely assigned and many of the remaining corresponded to duplicated sequences. They provide experimental evidence for the expression of 'hypothetical' genes ...