Protein Assay Manufacturers and Products

From the decades-old biuret reaction to the use of complicated fluorescent molecules, the search for an easy, reliable, and stable method for the quantitation of total protein in solution has gone on for more than 80 years. Although several famous, innovative, and useful techniques were developed over that time, the search continues.

Bob Vigna, marketing manager for industry leader Pierce Chemical Co. of Rockford, Ill., confirms this observation. "The use of protein assays is still a growing area for us," he says. Pierce has recently introduced its new Coomassie Dry Protein Assay Plate. The first protein assay available prepared in a ready-to-use 96-well plate format, the Coomassie Dry Assay uses a unique Bradford formulation stabilized as a glass within each well. Samples are simply added, mixed, and read on a microplate reader.

Typically, the conventional protein assay consists of the addition of a chemical...

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